Long-term preservation of transfecting activity of avian sarcoma proviral DNA.
نویسندگان
چکیده
The integrated proviral DNA of avian sarcoma virus (ASV) in host cell chromosomes has been isolated and stored in saline sodium citrate (SSC) solution or in 70% ethanol at 4 degrees C in a refrigerator over 4 years. This DNA was assayed by transfection of chick embryo cells(CEC). The biological activity of cellular transformation by the stored DNA was compared with that of a fresh isolate of the proviral DNA. The efficiency of the transfection by each DNA was almost the same.
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ورودعنوان ژورنال:
- Acta medica Okayama
دوره 35 5 شماره
صفحات -
تاریخ انتشار 1981